MYB elongation is regulated by the nucleic acid binding of NFekB p50 to the intronic stem-loop region

LA Pereira; HJ Hugo; J Malaterre; X Huiling; S Sonza; A Cures; DFJ Purcell; PA Ramsland; S Gerondakis; TJ Gonda; RG Ramsay

Journal article

MYB elongation is regulated by the nucleic acid binding of NFekB p50 to the intronic stem-loop region

LA Pereira, HJ Hugo, J Malaterre, X Huiling, S Sonza, A Cures, DFJ Purcell, PA Ramsland, S Gerondakis, TJ Gonda, RG Ramsay

Plos One | PUBLIC LIBRARY SCIENCE | Published : 2015

DOI: 10.1371/journal.pone.0122919

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Abstract

MYB transcriptional elongation is regulated by an attenuator sequence within intron 1 that has been proposed to encode a RNA stem loop (SLR) followed by a polyU tract. We report that NFekBp50 can bind the SLR polyU RNA and promote MYB transcriptional elongation together with NFekBp65. We identified a conserved lysine-rich motif within the Rel homology domain (RHD) of NFekBp50, mutation of which abrogated the interaction of NFekBp50 with the SLR polyU and impaired NFekBp50 mediated MYB elongation. We observed that the TAR RNA-binding region of Tat is homologous to the NFekBp50 RHD lysine-rich motif, a finding consistent with HIV Tat acting as an effector of MYB transcriptional elongation in a..

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University of Melbourne Researchers

Lloyd Pereira Author

Huiling Xu Author

Damian Purcell Author

Paul Ramsland Author

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

The National Health and Medical Research Council of Australia, https://www.nhmrc.gov.au/, 487922, RR, Australian Research Council, DP0985025, http://www.arc.gov.au/, RR and National Institutes of Health grant, 5-R21-AI-54158-2, http://www.nih.gov/, RR. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.